Christian Eriksen’s 40th-minute strike ended Southampton’s winning run of six games in all competitions as Spurs edged a close game at White Hart Lane.
The Tottenham midfielder found the back of the net with a crisp low finish towards the end of the first-half which Saints never recovered from, as the visitors failed to score in a game for the first time since mid-August.
Ronald Koeman’s team pushed hard for a response, with both Dušan Tadić and Victor Wanyama going closest to finding a way past goalkeeper Hugo Lloris, but the hosts held out to maintain their winning run over Saints since they returned to the Premier League.
Koeman went with an unchanged starting eleven from the team that began the win over Queens Park Rangers a week earlier, though Florin Gardos was fit enough to return for a place among the substitutes bench.
Spurs were also unchanged from the team which drew 1-1 at Arsenal, with Emmanuel Adebayor leading the line for the hosts.
The trademark fast-start to a game from Pochettino’s team saw the home side threatened as early as the third minute when defender Jan Vertonghen rose to head an effort from a corner towards the far post, where Ryan Bertrand was well-place to clear.
Saints responded well as they resisted the pressing style from Spurs, to slowly gain control of the contest, and in the 21st minute, the visitors were close to going in front when Morgan Schneiderlin met Sadio Mané’s cross from the left channel, but Vertonghen stood his ground to put in a vital block from close range.
Tottenham, who lost full-back Kevin Naughton to injury just past the half hour mark, withstood the pressure and came back at Saints to cause Koeman’s men - who were playing into the sunlight - a few problems to deal with. Fraser Forster was twice called upon to deny Erik Lamela shots towards the bottom corners, before the hosts grabbed the opening goal of the game five minutes before half-time.
The visitors appealed in vain, a possible handball during the build-up, but a simple one-two played by Adebayor and Eriksen, saw the former pulled away to the left, before feeding the ball into Eriksen who found the bottom left corner with an arrow of a shot.
Although Pellè saw a low shot from the edge of the area saved by Lloris, Spurs should perhaps have doubled their advantage just before the break when Adebayor played Chadli into a one-v-one situation with Forster, whom he beat, but the low shot came back off the inside of the post.
The last chance of the half saw Tadić step up for a low left footed curler of a free-kick which was saved by Lloris as the hosts held their slender lead at the interval.
After the break, Saints were denied the chance to test Lloris when Kaboul shoved Mané to the ground, with the defender booked for his challenge on the Senegalese winger.
On the hour, Bertrand recovered strongly to put a block on Chadli, but Saints continued to apply pressure at the other end of the field. A weak clearance from Kaboul fell invitingly towards Victor Wanyama, whose rising shot was brilliantly pushed away by Lloris.
Mané caused a few headaches for Spurs substitute Eric Dier, with Saints turning the screw when they made their first change of the game on 75 minutes as Shane Long replaced Wanyama.
Not too many chances arrived immediately thereafter, but the visitors should have done better on 83 when Bertrand crossed low into the area, and Mané fluffed his lines from six yards out.
Saints rallied in the closing stages against a tiring home side, but couldn’t find a way past Lloris, as the team’s long unbeaten run came to an end.
Tottenham team: Hugo Lloris, Danny Rose, Jan Vertonghen, Younes Kaboul (c), Kyle Naughton (Eric Dier 32), Christian Eriksen (Mousa Dembele 81), Etienne Capoue, Erik Lamela (Harry Kane 90), Nacer Chadli, Ryan Mason, Emmanuel Adebayor
Unused substitutes: Michel Vorm, Federico Fazio, Andros Townsend, Roberto Soldado
Goal: Eriksen (40)
Booked: Kaboul, Lamela
Southampton team: Fraser Forster, Nathaniel Clyne, José Fonte (c), Toby Alderweireld, Ryan Bertrand, Morgan Schneiderlin, Victor Wanyama (Shane Long 75), Steven Davis, Dušan Tadić, Sadio Mané, Graziano Pellè
Unused substitutes: Kelvin Davis, Florin Gardos, Matt Targett, Jack Cork, Harrison Reed, Emmanuel Mayuka
Referee: Mike Jones