Having helped Stuart Pearce's side top their qualification group, Saints defender Clyne has been named in a 26-man party for their decisive two-legged play-off to determine whether they qualify for next summer's finals.
For the first leg, England host Serbia at Norwich's Carrow Road stadium on Friday 12th October with a 6pm kick-off.
The return fixture takes place four days later on Tuesday 12th October at the Stadion Mladost, home of FK Napredak Kruševac. Kick off takes place at 6pm local time, which is 5pm BST.
The winners of the seven play-off matches will qualify for the 2013 UEFA European Under-21 Football Championship, which takes place in Israel next June.
Clyne currently has three caps for the Young Lions, following up his first appearances in 2011 by featuring in last month's win over Norway.