Clyne is hoping to be involved as England's Under-21s take on Romania in a friendly at Adams Park as they prepare for this summer's UEFA European Under-21 Championships in Israel.
Ward-Prowse and Chambers are with the England Under-19 squad as they face their Turkish counterparts in Telford ahead of May's Elite Group qualifying matches.
A total of 12 Southampton players were called into their respective national squads ahead of this week, though three – Luke Shaw, Danny Fox and Lloyd Isgrove – have since withdrawn through injury.
Here's a full run-down of who's playing where and when:
Thursday 21st March 2013, 7pm GMT
NATHANIEL CLYNE: ENGLAND U21 vs Romania U21 – Friendly (Adams Park – Wycombe, England)
Thursday 21st March 2013, 7.30pm GMT
JAMES WARD-PROWSE & CALUM CHAMBERS: ENGLAND U19 vs Turkey U19 – Friendly (New Bucks Head Stadium – Telford, England)
Friday 22nd March 2013, 4.05pm GMT
MAYA YOSHIDA: JAPAN vs Canada – Friendly (Khalifa International Stadium – Doha, Qatar)
Friday 22nd March 2013, 5pm GMT
VEGARD FORREN: NORWAY vs Albania – 2014 FIFA World Cup UEFA Qualifying Group E (Ullevaal Stadion – Oslo, Norway)
Friday 22nd March 2013, 6.45pm GMT
ARTUR BORUC: POLAND vs Ukraine – 2014 FIFA World Cup UEFA Qualifying Group H (Stadion Narodowy – Warsaw, Poland)
Friday 22nd March 2013, 7.45pm GMT
STEVEN DAVIS: NORTHERN IRELAND vs Russia – 2014 FIFA World Cup UEFA Qualifying Group F (Windsor Park – Belfast, Northern Ireland)
Friday 22nd March 2013, 10pm GMT
GASTÓN RAMÍREZ: URUGUAY vs Paraguay – 2014 FIFA World Cup CONMEBOL Qualifying (Estadio Centenario – Montevideo, Uruguay)
Sunday 24th March 2013, 1pm GMT
EMMANUEL MAYUKA: Lesotho vs ZAMBIA – 2014 FIFA World Cup CAF Qualifying Second Round Group D (Setsoto Stadium – Maseru, Lesotho)
Monday 25th March 2013, 7.30pm GMT
NATHANIEL CLYNE: ENGLAND U21 vs Austria U21 – Friendly (Amex Stadium – Brighton, England)
Tuesday 26th March 2013, 4pm GMT
MAYA YOSHIDA: Jordan vs JAPAN - 2014 FIFA World Cup AFC Qualifying Fourth Road Group B (King Abdullah Stadium – Amman, Jordan)
Tuesday 26th March 2013, 6.45pm GMT
ARTUR BORUC: POLAND vs San Marino – 2014 FIFA World Cup UEFA Qualifying Group H (Stadion Narodowy – Warsaw, Poland)
Tuesday 26th March 2013, 7.45pm GMT
STEVEN DAVIS: NORTHERN IRELAND vs Israel – 2014 FIFA World Cup UEFA Qualifying Group F (Windsor Park – Belfast, Northern Ireland)
Tuesday 26th March 2013, 11.30pm GMT
GASTÓN RAMÍREZ: Chile vs URUGUAY – 2014 FIFA World Cup CONMEBOL Qualifying (Estadio Monumental David Arellano – Santiago, Chile)